おすすめの製品
由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
分子量
antigen ~35 kDa
化学種の反応性
human
テクニック
immunohistochemistry: 1:250 using human cerebral cortex sections
western blot: 1:250-1:500 using PC-12 cell lysate
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... EIF2S1(1965)
詳細
The eukaryotic translation initiation factor 2 subunit α (EIF2A) gene is mapped to human chromosome 14q23.3. The eukaryotic translation initiation factor 2 (eIF2), a multimeric protein is composed of three subunits termed α, β, and γ.
特異性
Anti-eIF2α (also known as eIF-2A) recognizes human eIF2α.
アプリケーション
Anti-eIF2α antibody produced in rabbit has been used in western blotting.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.
Anti-eIF2α antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:250-1:500 using PC-12 cell lysate. It was used as a primary antibody to detect eIF2α in a study.
生物化学的／生理学的作用
Increased eIF2α phosphorylation has been observed in Alzheimer′s disease. Viral infections are also known to induce eIF2α phosphorylation.
The main role of eIF2 complex is to transfer Met-tRNAi in the presence of GTP to the 40S ribosomal subunit during the initiation of translation. Regulation of the initiation of translation at the level of eIF2 involves the phosphorylation of eIF2α at a conserved Ser51 residue. This leads to inhibition of eIF2β activity, thus reducing guanine nucleotide exchange and consequently, the translation rate. Protein synthesis inhibition at the level of eIF2α phosphorylation is linked to a variety of cell stress signaling pathways via specific kinases. Phosphorylation of eIF2α reduces the rate of translation and gives the cell time to correct the stress damage, and selectively enhance the translation of genes important for stress remedy.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E0157-BULK:
IXO14403:
E0157-VAR:
E0157-200UL:
E0157-25UL:
最新バージョンのいずれかを選択してください：
R C Wek et al.
Biochemical Society transactions, 34(Pt 1), 7-11 (2005-10-26)
In response to environmental stresses, a family of protein kinases phosphorylate eIF2 (eukaryotic initiation factor 2) to alleviate cellular injury or alternatively induce apoptosis. Phosphorylation of eIF2 reduces global translation, allowing cells to conserve resources and to initiate a reconfiguration
R C Wek
Trends in biochemical sciences, 19(11), 491-496 (1994-11-01)
Phosphorylation of eukaryotic initiation factor-2 (eIF-2) is an important mechanism regulating general translation initiation. Two mammalian eIF-2 kinases, the double-stranded-RNA-dependent kinase (PKR) and heme-regulated inhibitor kinase (HRI), have been characterized by sequencing, revealing shared sequence and structural features distinct from
Christopher G Proud
Seminars in cell & developmental biology, 16(1), 3-12 (2005-01-22)
Eukaryotic initiation factor eIF2 and its 'exchange factor' eIF2B play a key role in the regulation of protein synthesis in eukaryotes from yeast to mammals. Phosphorylation of eIF2 inhibits eIF2B and thus translation initiation. Four eIF2 kinases are now known
Rita Spilka et al.
Cancer letters, 340(1), 9-21 (2013-07-09)
Eukaryotic gene expression is a complicated process primarily regulated at the levels of gene transcription and mRNA translation. The latter involves four main steps: initiation, elongation, termination and recycling. Translation regulation is primarily achieved during initiation which is orchestrated by
Anton A Komar et al.
International journal of molecular sciences, 21(6) (2020-03-21)
Initiation of protein synthesis in eukaryotes is a complex process requiring more than 12 different initiation factors, comprising over 30 polypeptide chains. The functions of many of these factors have been established in great detail; however, the precise role of
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）