CMC0008

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

• UNSPSCコード: 12352200

特性

• product name: STEADY Chemically Competent Cells, for plasmid production using unstable DNA
• 由来生物: Escherichia coli
• グレード: for molecular biology
• 形状: buffered aqueous solution
• 成長モード: adherent or suspension
• 形態: rod shaped
• テクニック: microbiological culture: suitable
• 細胞形質転換: competent cell type: chemically competent; transformation efficiency: >1 x 10⁸ cfu/μg
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

特徴および利点

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chemi) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

構成

• STEADY chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

安全性情報

• 保管分類コード: 10 - Combustible liquids