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Merck

CLL1033

Sigma-Aldrich

U2OS GFP-LMNB1

human female bone (Disease source: Osteosarcoma)

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About This Item

UNSPSCコード:
41106514
NACRES:
NA.81

product name

U2OS GFP-LMNB1,

由来生物

human female bone (Source Disease: Osteosarcoma)

品質水準

OMIMアクセッション番号

保管温度

−196°C

遺伝子情報

human ... lamin B1(4001)

詳細

LMNB1 (lamin B1) is a B type lamin protein. LMNB1 is abundantly expressed in various cell types. This gene is located on human chromosome 5q23. The nuclear lamina, an intermediate filament meshwork, is made of LmnB1, LmnB2, A type (LmnA/C) and associated inner nuclear membrane proteins.
This product was derived from ATCC Cat. No. HTB-96.
U2OS GFP-LMNB1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.

アプリケーション

This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of LaminB1. Fluorescence imaging shows the characteristic LaminB1 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage

生物化学的/生理学的作用

Lamin B1 (LMNB1) is essential for proper chromosome condensation in interphase nuclei. It also participates in the distribution of heterochromatin and modulation of gene expression and splicing. Mutations in LMNB1 (lamin B1) gene results in the adult onset demyelinating disease autosomal dominant leukodystrophy (ADLD).
Nuclear lamin B1 encoded by LMNB1 gene is an intermediate filament protein of the nuclear envelope. The protein has transcriptional co-regulatory activity. It plays a key role in DNA replication, cellular aging and stress responses. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC) patients. Thus, LMNB1 can be used as a potential biomarker for HCC.

特徴および利点

染色体 5q23.2にGFPタグを付加
The U2OS cells are adherent, with a doubling time of approx. 29 hours.

構成

U2OS GFP-LMNB1細胞

品質

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

調製ノート

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164).

法的情報

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

関連製品

製品番号
詳細
価格

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

CLL1033-1VL:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

The role of lamin B1 for the maintenance of nuclear structure and function.
Camps J, et al.
Nucleus (Austin, Tex.), 6(1), 8-14 (2015)
Nuclear envelope rupture is induced by actin-based nucleus confinement.
Hatch EM and Hetzer MW
The Journal of Cell Biology, 215(1), 27?36-27?36 (2016)
Defects of lipid synthesis are linked to the age-dependent demyelination caused by lamin B1 overexpression.
Rolyan H, et al.
The Journal of Neuroscience, 35(34), 12002-12017 (2015)
Structural Organization of the Human Gene (LMNB1) Encoding Nuclear Lamin B1
Lin F, et al.
Genomics, 27(2), 230-236 (1995)
Circulating Lamin B1 (LMNB1) Biomarker Detects Early Stages of Liver Cancer in Patients
Sun S, et al.
Journal of Proteome Research, 9(1), 70-78 (2009)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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