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product name
U2OS GFP-TUBA1B,
由来生物
human female bone (Source Disease: Osteosarcoma)
品質水準
OMIMアクセッション番号
保管温度
−196°C
遺伝子情報
human ... alpha tubulin 1B(10376)
詳細
K-α1 tubulin/ tubulin α 1b (TUBA1B) is a microtubule protein. It is a ~50 kDa protein in its poly glycosylated, post-translational form. TUBA1B is present in cells as one of the six different isotypes. This gene is located on human chromosome 12q13.12.
This product corresponds to ATCC Cat. No. HTB-96.
U2OS GFP-TUBA1B are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.
アプリケーション
This product is a human U2OS cell line in which the genomic TUBA1B gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of tubulin. Fluorescence imaging shows that the tubulin can polymerize to form the characteristic pattern of microtubules. Addition of vincristine to this cell line shows the expected depolymerization of microtubules. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
生物化学的/生理学的作用
K-α1 tubulin/ tubulin α 1b (TUBA1B) participates in several cellular activities like GTP binding, GTPase activity, maintaining cellular structure in the form of microtubules and microtubule-based intracellular movement. It plays a crucial role in polymerization and depolymerization during cell replication and division. TUBA1B is overexpressed in hepatocellular carcinoma (HCC) tumor tissues and proliferating HCC cells.
Tubulin α 1b (TUBA1B) plays an important role in dynamic process of polymerization and depolymerization during cell replication and division. Elevated expression of the gene has been observed in hepatocellular carcinoma (HCC) tumor tissues and proliferating HCC cells. This upregulated expression of the gene leads to poor prognosis and chemotherapy resistance in HCC.
特徴および利点
染色体12q13.12にGFPタグを付加
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
構成
U2OS GFP-TUBA1B細胞
品質
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
調製ノート
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
法的情報
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CLL1031-1VL:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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De novo production of K-α1 tubulin-specific antibodies: role in chronic lung allograft rejection.
Journal of Immunology, 180(7), 4487-4494 (2008)
Increased α-tubulin1b expression indicates poor prognosis and resistance to chemotherapy in hepatocellular carcinoma.
Digestive Diseases and Sciences, 58(9), 2713-2720 (2013)
Up-regulation of TUBA1B promotes astrocyte proliferation after spinal cord injury in adult rats.
International Journal of Clinical and Experimental Pathology, 10(2), 1094-1103 (2017)
A revised nomenclature for the human and rodent alpha-tubulin gene family.
Genomics, 90(2), 285-289 (2007)
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