HT2 Clone A5E

92021401, mouse blood, Lymphoblast

• UNSPSCコード: 41106514
• 由来生物: mouse blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblast
• 製品: Not specified
• 受容体: Interleukin 2 (IL-2), Interleukin 4 (IL-4)

特性

• 製品名: HT2 Clone A5E, 92021401
• 由来生物: mouse blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblast
• 製品: Not specified
• 受容体: Interleukin 2 (IL-2), Interleukin 4 (IL-4)
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse helper T cell

細胞株の説明

Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).

アプリケーション

IL-2 assays

培地

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g

その他情報

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