MG-63 Cell Line human

86051601, human bone, Fibroblast

• 別名: MG63 Cells
• UNSPSCコード: 41106514

特性

• product name: MG-63 Cell Line human, 86051601
• 由来生物: human bone
• 詳細: Human osteosarcoma
• 成長モード: Adherent
• 核型: Modal no. 66; hypotriploid
• 形態: Fibroblast
• 製品: Inducible interferon
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human osteosarcoma

細胞株の説明

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

アプリケーション

Human MG-63 cell line has been used:

• to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells
• to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells
• as a positive control for immunostaining procedure
• to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells

Interferon-induction studies

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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