SP2/O-Ag14 Cell Line murine

85072401, Non-producing hybridoma

• 別名: Mouse Cell Line
• UNSPSCコード: 12352207

特性

• 由来生物: mouse spleen
• 包装: tube of 5 μg 85072401-DNA-5UG; pkg of vial of cells 85072401-1VL
• 成長モード: Suspension
• 核型: Not specified
• 形態: Not specified
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse x Mouse myeloma, non-producing

細胞株の説明

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

アプリケーション

Fusion partner

培地

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. HAT sensitive.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.