CHO-K1 Cell Line from hamster

85051005, hamster ovary, Epithelial

• 別名: CHO cell clone K1 Cells, CHOK1 Cells
• UNSPSCコード: 41106514

特性

• product name: CHO-K1 Cell Line from hamster, 85051005, from hamster ovary
• 由来生物: hamster ovary
• 成長モード: Adherent
• 核型: Hypodiploid, modal no. 20
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Hamster Chinese ovary

細胞株の説明

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

アプリケーション

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

培地

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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