GPNT

9061601, rat brain, Fusiform

• UNSPSCコード: 41106514
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fusiform
• 製品: GPNT cells express tight junction proteins
• 受容体: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I

特性

• 製品名: GPNT, from rat brain, 09061601
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fusiform
• 製品: GPNT cells express tight junction proteins
• 受容体: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice

詳細

細胞株の由来

An immortalised Lewis rat brain vascular endothelial cell line

細胞株の説明

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

DNAプロファイル

特記事項なし

培地

Hams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 10hrs.

その他情報

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免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.