108CC5-TG-4

8062546, Mouse x Rat Hybridoma nerve, Neuronal

• UNSPSCコード: 41106514

特性

• product name: 108CC5-TG-4, 08062546
• 由来生物: Mouse x Rat Hybridoma nerve
• 成長モード: Adherent
• 核型: Not specified
• 形態: Neuronal
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse neuroblastoma x Rat glioma hybrid

細胞株の説明

A 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid

アプリケーション

Used as a neuronal Model

培地

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

その他情報

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