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product name
Sf21 TitreHigh AC free, 05030202
由来生物
insect ovary
成長モード
Adherent
核型
Not specified
形態
Spherical
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Spodoptera frugiperda pupal ovarian tissue, serum-free
細胞株の説明
This cell line is a derivative of the Sf21 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf21 (Spodoptera frugiperda) insect cell line. The cell line Sf21 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf21 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.
アプリケーション
Protein expression using the Baculovirus vector system, serum-free
培地
TiterHigh™ Sf Insect Medium (Sigma I5408) no supplements.
継代と培養方法
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.
その他情報
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法的情報
TiterHigh is a trademark of Sigma-Aldrich Co. LLC
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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