Sf21

5022801, insect ovary, Spherical

• UNSPSCコード: 41106514

特性

• product name: Sf21, 05022801
• 由来生物: insect ovary
• 成長モード: Adherent
• 核型: Not specified
• 形態: Spherical
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Spodoptera frugiperda pupal ovarian tissue

細胞株の説明

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

アプリケーション

Protein expression using the Baculovirus vector system

培地

SF90011 + 10% Foetal Bovine Serum (FBS)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

その他情報

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