MOCHA

2111901, mouse skin, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: MOCHA, 02111901
• 由来生物: mouse skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Skin Fibroblast

細胞株の説明

Mocha is a naturally occuring mouse mutant with a null mutation in the delta subunit of AP-3 complex. In Mocha cells, the ß3 amd μ3 subunits coassmeble into a heterodimer whereas the sigma 3 subunit remains monomeric. The mouse has a similar phenotype to the human genetic disorder, Hermansky Pudlak Syndrome (HPS). In both mice and humans, AP-3 deficiency results in hypopigmentation because of abnormalities in melanosomes. The Mocha cell line can be used to investigate the assembly of AP-3 complexes and AP-3 function.

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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