CASP8C

カスパーゼ8 比色アッセイキット

• UNSPSCコード: 12352202
• NACRES: NA.84

特性

• 輸送温度: dry ice
• 品質水準: 200
• 保管温度: −20°C
• 遺伝子情報: human ... CASP8(841)

詳細

詳細

Caspases (cysteine-requiring aspartate proteases) belong to a highly conserved family of cysteine proteases with specificity for aspartic acid residues found in their substrates. Caspases play a central role in apoptosis. Inhibition of caspases prevents the cell death process. Currently, there are 13 caspases recognized in mammals. Caspases are classified as initiators and effectors. Caspase 8 belongs to the class of initiators. It possesses a prodomain at the terminal and contains the death effector domain which serves as protein-protein interaction motif for caspase 8. With respect to substrate specificity, caspase 8 is classified as a member of group III. It specifically recognizes the (I/L/V) EXD sequence on the substrate.

アプリケーション

The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:

• purified caspase 8 for a positive control
• caspase 8 inhibitor
• p-NA fluorescent standard

数量

キットには96ウェルプレートで反応容量100 μLのとき100検体分に使用できる試薬が入っています。

キットの構成要素のみ

• N-Acetyl-Ile-Glu-Thr-Asp-al, ≥97% (HPLC) 100 μL
• Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL
• 10X Assay Buffer 5 mL
• 5X Lysis Buffer 5 mL

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable