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由来生物
donkey
品質水準
結合体
alkaline phosphatase conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
フォーム
buffered aqueous glycerol solution
テクニック
direct ELISA: 1:30,000
western blot: 1:30,000
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
詳細
Affinity isolated antibody is obtained from anti-sheep IgG antiserum by immunospecific purification which removes essentially all donkey serum proteins, including immunoglobulins, which do not specifically bind to sheep IgG. Anti-Sheep IgG is conjugated to alkaline phosphatase by protein cross-linking with 0.2% glutaraldehyde. Specificity of the antiserum is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, the antiserum reacts specifically with normal sheep serum and sheep IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the product followed by diffusion against anti-horse IgG and anti-donkey whole serum results in single arcs of precipitation.
IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody binds to all sheep immunoglobulins.
特異性
normal sheep serum and sheep IgG
免疫原
Purified sheep IgG
アプリケーション
Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Solid-Phase Decapeptide Assays were performed using alkaline phosphatase conjugated donkey anti-sheep IgG at 1:1000 for 2-3 hours at room temperature.
The anibody may be used in applications such as immunoblotting, dot blot and direct ELISA at a dilution of 1:30,000
物理的形状
Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 50% glycerol, 1% bovine serum albumin, and 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A5187-.5ML:
A5187-1ML:
A5187PROC:
A5187-VAR:
A5187-BULK:
A5187-.25ML:
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