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由来生物
mouse
品質水準
結合体
peroxidase conjugate
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
secondary antibodies
クローン
RG-16, monoclonal
フォーム
lyophilized powder
交差性
rabbit
以下との反応禁止
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
包装
vial of 0.5 mL
テクニック
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue
western blot (chemiluminescent): 1:160,000
アイソタイプ
IgG1
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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関連するカテゴリー
詳細
Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
特異性
ウサギIgとのみ結合します。還元されたウサギIgとは反応しません。
アプリケーション
Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:
- western blotting
- immunoblotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
物理的形状
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
調製ノート
Avrameas, S.らによる二段階グルタルアルデヒド法で作製しています(Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978))。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 2
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A2074-1VL-PW:
A2074-1VL:4548173184999
A2074-VAR:
A2074-BULK:
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