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由来生物
goat
品質水準
結合体
alkaline phosphatase conjugate
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
secondary antibodies
クローン
polyclonal
交差性
rabbit
テクニック
direct ELISA: 1:20,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:100,000
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains, held together by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
特異性
Goat polyclonal anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody reacts with rabbit IgG in vitro, in rabbit serum and biological fluids.
免疫原
pooled normal rabbit serum
アプリケーション
アルカリホスファターゼ結合ヤギ抗ウサギIgGを0.05M炭酸／重炭酸バッファー（Ph 9.6）中に1:5000で希釈して二次抗体としたELISAによって、PADI4によるアンチトロンビンのシトルリン化が分析されました。
Anti-Rabbit IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat has been used in western blotting and enzyme-linked immunosorbent assay (ELISA).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
生物化学的／生理学的作用
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region contributes for the development of therapeutic antibody.
物理的形状
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
法的情報
CDP-Star is a registered trademark of Tropix, Inc.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A0418-VAR:
A0418-BULK:
A0418-1ML:
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Assessing carnivorous plants for the production of re-combinant proteins
Miguel S, et al.
Frontiers in Plant Science, 10, 793-793 (2019)
Cellular casein kinase 2 and protein phosphatase 2A modulate replication site assembly of bluetongue virus
Mohl BP and Roy P
Test, 291(28), 14566-14574 (2016)
C H Little et al.
Infection and immunity, 68(7), 3840-3847 (2000-06-17)
Immunoglobulin G (IgG) and T-cell-derived antigen binding molecules (TABM) specific to whole Candida extract and to Candida-derived mannans prepared by both the cetryltrimethylammonium bromide (CTAB) and alkaline degradation (PEAT) methods were measured in the sera of women with vulvovaginal candidiasis
Sissi Miguel et al.
Communications biology, 3(1), 673-673 (2020-11-15)
The synthesis of 3,5-dicaffeoylquinic acid (3,5-DiCQA) has attracted the interest of many researchers for more than 30 years. Recently, enzymes belonging to the BAHD acyltransferase family were shown to mediate its synthesis, albeit with notably low efficiency. In this study
Carmel M McVicar et al.
Fertility and sterility, 81 Suppl 1, 767-774 (2004-03-17)
To determine the incidence of Fas positivity and DNA double-strand breaks (DSB) as indicators of early- and late-stage apoptosis in ejaculated sperm. Fas positivity was assessed by flow cytometry and DSB by the neutral Comet assay. Andrology Laboratory, Royal Maternity
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