93337

トリズマ^®酢酸塩

BioUltra, ≥99.0% (NT)

• 別名: トリス 酢酸塩, トリス(ヒドロキシメチル)アミノメタン 酢酸塩
• 化学式: NH₂C(CH₂OH)₃ · CH₃COOH
• CAS番号: 6850-28-8
• 分子量: 181.19
• Beilstein: 3702918
• EC Number: 229-939-6
• MDL番号: MFCD00038951
• UNSPSCコード: 12161700
• PubChem Substance ID: 57653535
• NACRES: NA.25

特性

• 製品種目: BioUltra
• アッセイ: ≥99.0% (NT)
• フォーム: crystalline powder
• 不純物: insoluble matter, passes filter test
• 強熱残分: ≤0.2%
• 損失: ≤0.2% loss on drying, 20 °C (HV)
• pH: 6.0-7.0 (25 °C, 0.5 M in H₂O)
• 溶解性: H₂O: 0.5 M at 20 °C, clear, colorless
• 微量陰イオン: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• 微量陽イオン: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV吸収: λ: 260 nm A_max: 0.07; λ: 280 nm A_max: 0.05
• SMILES記法: CC(O)=O.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)
• InChI Key: PIEPQKCYPFFYMG-UHFFFAOYSA-N

詳細

アプリケーション

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

その他情報

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferasecitation; Assay of glutamate bindingcitation

ホタルルシフェラ-ゼを用いたATPアッセイの感度を最大にするために推奨されるバッファ-です。グルタミン酸結合アッセイに用いられます。

法的情報

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)