69898

ブロモビマン

suitable for fluorescence, BioReagent, ≥95% (HPCE)

• 別名: モノブロモビマン
• 実験式（ヒル表記法）: C₁₀H₁₁BrN₂O₂
• CAS番号: 71418-44-5
• 分子量: 271.11
• Beilstein: 4430959
• MDL番号: MFCD00036951
• UNSPSCコード: 12171500
• PubChem Substance ID: 57652223
• NACRES: NA.32

特性

• 製品種目: BioReagent
• アッセイ: ≥95% (HPCE)
• 形状: solid
• mp: 152-154 °C (lit.)
• 溶解性: DMF: soluble; DMSO: soluble; acetonitrile: soluble; methanol: soluble
• 蛍光検出: λ_ex 390 nm; λ_em 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione); λ_ex 398 nm
• 適合性: suitable for fluorescence
• 保管温度: 2-8°C
• SMILES記法: CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
• InChI: 1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
• InChI Key: AHEWZZJEDQVLOP-UHFFFAOYSA-N

詳細

詳細

Bromobimane, also called probe for thiols, is a fluorescent reagent activated upon a photolysis reaction. Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH. And, upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

アプリケーション

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.

特徴および利点

The benefits of using Bromobimane for the analysis of low molecular weight thiols are: Highly selective.

• Rapid reactivity.
• Ease of separation of derivatives by reverse HPLC.
• Ability to penetrate cells.

包装

底の開いたガラス瓶内容物は内部に挿入され接着された円錐部に入っています。

その他情報

チオ-ル特異的蛍光試薬

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)