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由来生物
human
品質水準
リコンビナント
expressed in E. coli
詳細
0.1 mg recombinant human CD2 in 20 mM Tris-HCl buffer, pH 8.0, containing NaCl, KCl, EDTA, L-arginine, DTT, and glycerol
無菌性
Filtered sterilized solution
アッセイ
≥90% (SDS-PAGE)
フォーム
liquid
包装
pkg of 100 μg
濃度
0.5 mg protein/mL
テクニック
cell culture | mammalian: suitable
アクセッション番号
NP_001758
輸送温度
dry ice
保管温度
−20°C
遺伝子情報
human ... CD2(914)
アプリケーション
Coating a plate well (6 well plate) with this recombinant CD2 protein in a T cell specific medium at 1-10 μg/well allows for human T cell differentiation studies in vitro.
Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.
Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.
シーケンス
MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD
調製ノート
The full-length extracellular domain of the human CD2 gene (25-209 aa) was constructed with 31 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
5086-100UG:
5086-VAR:
5086-BULK:
最新バージョンのいずれかを選択してください：
Clare M Baecher-Allan et al.
Journal of immunology (Baltimore, Md. : 1950), 186(6), 3317-3326 (2011-02-09)
Studying the activity of homogeneous regulatory T cell (Treg) populations will advance our understanding of their mechanisms of action and their role in human disease. Although isolating human Tregs exhibiting low expression of CD127 markedly increases purity, the resulting Treg
B Schraven et al.
Nature, 345(6270), 71-74 (1990-05-03)
At least two membrane receptors have been defined through which human T lymphocytes can be induced to proliferate and differentiate, namely the CD3-Ti antigen receptor complex and the CD2 molecule. Monoclonal antibodies directed at either CD2 or CD3 induce intracellular
Eun-Ok Kim et al.
The Journal of biological chemistry, 285(53), 41755-41764 (2010-09-04)
Human natural killer (NK) cells express an abundant level of 2B4 and CD2 on their surface. Their counter-receptors, CD48 and CD58, are also expressed on the NK cell surface, raising a question about the functional consequences of potential 2B4/CD48 and
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