SAB4200545

モノクロナール抗パルブアルブミン

clone PARV-19, purified from hybridoma cell culture

• 別名: Monoclonal Anti-ALPHA-PARVALBUMIN, Monoclonal Anti-PALB1, Monoclonal Anti-PV, Monoclonal Anti-PVALB (includes EG:19293), Monoclonal Anti-Parv, Monoclonal Anti-Pva
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: PARV-19, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 12 kDa
• 交差性: rat, human
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat cerebellum.; indirect immunofluorescence: 10-20 μg/mL using BLO-11 cells.; western blot: 1-2 μg/mL using mouse muscle extracts.
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PVALB(5816) ; rat ... PVALB(25269)

詳細

詳細

Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the hybridoma PARV-19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified frog muscle parvalbumin. Parvalbumin (PV) is a member of the family of Ca²⁺-binding molecules. It carries three helix-loop-helix EF-hand Ca²⁺ binding motifs, with the two C-terminal motifs.

特異性

Monoclonal Anti-Parvalbumin recognizes parvalbumin in a Ca2+ -ion dependent manner

免疫原

purified frog muscle parvalbumin. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

アプリケーション

Anti-Parvalbumin antibody, Mouse monoclonal has been used in:

• immunohistochemistry
• immunofluorescence staining
• immunolabeling
• to label brain slices for perineuronal net staining
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生物化学的／生理学的作用

Ca²⁺ participates in cell motility, release of neurotransmitters, membrane permeability, excitation−contraction of muscles, cell secretory procedures, and transmission of nerve impulses. Intracellular Ca²⁺ exchangers are essential to modulate these processes efficiently. Parvalbumin (PV), a calcium-binding protein, can regulate the intracellular calcium dynamics in neurons. Hence it may have an impact on facilitation and depression at synapses. Interestingly, PV deficiency alters neuronal activity, a key mechanism leading to epileptic seizures or Parkinson.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable