V0132

Rat Vitronectin

from rat plasma, powder, suitable for cell culture

• 別名: 血清拡散因子
• CAS番号: 83380-82-9
• MDL番号: MFCD00132575
• UNSPSCコード: 12352202
• NACRES: NA.75

特性

• 製品名: ビトロネクチン ラット血漿由来, lyophilized powder, BioReagent, suitable for cell culture
• 由来生物: rat plasma
• 品質水準: 200
• 製品種目: BioReagent
• フォーム: lyophilized powder
• 分子量: 75 kDa
• 包装: pkg of 50 μg
• テクニック: cell culture | mammalian: suitable
• 表面被覆率: 0.1 μg/cm²
• 溶解性: water: soluble 1.00 mL, clear, colorless
• UniProtアクセッション番号: Q3KR94
• 輸送温度: dry ice
• 保管温度: 2-8°C
• 遺伝子情報: rat ... Vtn(29169)

詳細

詳細

Vitronectin, also known as epibolin and S protein, is a 75 kDa protein. It consists of three glycosylation sites. Vitronectin is mainly expressed in plasma.

アプリケーション

細胞培養基質として0.1 μg/cm²での使用が推奨されます。接着の至適条件は、各細胞株や用途によって異なります。

Vitronectin has been used as a constituent of serum free media for culturing skeletal muscle and for growth and myelination of motoneurons by Schwann cells. It has also been used as a constituent of enriched co-culture media for culturing rat spinal cord.

生物化学的／生理学的作用

Vitronectin inhibits the membrane cytolytic attack complex of the complement system. It interacts with glycosaminoglycans, collagen, plasminogen and the urokinase-receptor. Vitronectin binds to heparin, thrombin and antithrombin III during coagulation and may play a role in the coagulation pathway. It acts a connector between cell adhesion and physiological proteolysis. Vitronectin in association with integrins αvβ3, αvβ5, αvβ1, αIIbβ3, αvβ6 and αvβ8 stimulates cell adhesion, spreading and migration.

構成

ビトロネクチンと結合するインテグリン受容体を有する細胞には、血小板、内皮細胞、メラノ-マ細胞、骨肉腫などがあります。

注意

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

調製ノート

水で溶解してください(1 mL/バイアル)。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)