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由来生物
microbial
アッセイ
≥90%
フォーム
liquid
濃度
100 mg/mL in water
色
faint yellow to dark brown
抗生物質活性スペクトル
Gram-negative bacteria
Gram-positive bacteria
mycobacteria
neoplastics
作用機序
DNA synthesis | interferes
enzyme | inhibits
保管温度
−20°C
詳細
Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.
アプリケーション
Novobiocin has been used:
- in cell culture systems to treat mycoplasma contaminations.[3]
- in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]
生物化学的／生理学的作用
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II
Activity spectrum: Active against gram-positive and gram-negative bacteria
Activity spectrum: Active against gram-positive and gram-negative bacteria
特徴および利点
- Ready available solution reduce the need for preparation time
- Commonly used in Cell Biology and Biochemical applications
- High quality antibiotic suitable for mulitple research applications
調製ノート
Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.
その他情報
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シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SBR00025-BULK:
SBR00025-1ML-PW:
SBR00025-1ML:
SBR00025-VAR:
最新バージョンのいずれかを選択してください：
Environmentally constrained mutation and adaptive evolution in Salmonella.
Massey, R. C., Rainey, P. B., Sheehan, B. J., Keane, O. M., & Dorman, C. J.
Current Biology, 9, 1477-1481 (1999)
Biosynthesis of novobiocin by Streptomyces niveus.
Kominek, L. A.
Antimicrobial Agents and Chemotherapy, 1, 123-134 (1972)
Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors.
Kusuma, B. R., Duerfeldt, A. S., & Blagg, B. S.
Bioorganic & Medicinal Chemistry Letters, 21, 7170-7174 (2011)
Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Novobiocin antagonism of amastigotes of Trypanosoma cruzi growing in cell-free medium.
Pate, P. G., Wolfson, J. S., McHugh, G. L., Pan, S. C., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 29, 426-431 (1986)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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