39465

ゼラチン from porcine skin

suitable for microbiology, ultrahigh gel strength

• CAS番号: 9000-70-8
• EC Number: 232-554-6
• MDL番号: MFCD00081638
• UNSPSCコード: 41106212
• NACRES: NA.85

特性

• 由来生物: Porcine
• 品質水準: 100
• 形状: powder
• 品質: ultrahigh gel strength
• シェルフライフ: limited shelf life, expiry date on the label
• 損失: 9.5-12.5% loss on drying
• 透過率: 450 nm, ≥85%; 620 nm, ≥95%
• pH: 5.20-5.60
• 導電率: 120-190 μS/cm at 30 °C (1%)
• 粘度: 5.10-5.80 mPa.s
• ゲル強度: 280-302 g BloomAOAC
• 溶解性: H₂O: 67 mg/mL at 60 °C
• 微量陽イオン: Ca: ≤100 mg/kg
• アプリケーション: microbiology

詳細

アプリケーション

This product is recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

構成

ゼラチンは、コラーゲン中に存在する平均分子量が大きい水溶性タンパク質の不均一な混合物です。 水中で適切な皮膚、腱、靭帯、骨などを煮沸することでタンパク質が抽出されます。 A型ゼラチンは酸硬化組織由来です。 B型ゼラチンは石灰硬化組織由来です。

注意

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

調製ノート

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable