QIA39

FragEL DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme

• 別名: TUNEL Assay
• UNSPSCコード: 41116133
• NACRES: NA.51

特性

• 使用法: sufficient for 50 tests
• 品質水準: 100
• 化学種の反応性（ホモロジーによる予測）: all
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: flow cytometry: suitable
• 入力: sample type cell suspension(s); sample type frozen section(s); sample type paraffin section(s); sample type fixed-cell preparation(s)
• 検出方法: fluorometric
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.

Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.

構成

Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

規格

Assay Time: 2 h

原理

The Calbiochem Fluorescein-FragEL DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, in cell preparations fixed on slides, or in cell suspensions. Detection and analysis of labeling is possible by flow cytometry or fluorescence microscopy.

保管および安定性

Fluorescein-FragEL kit components are shipped on dry ice. Upon receipt, store kit at -20°C in a non-frost-free freezer. To avoid reagent loss in tube caps, briefly pulse spin all thawed solutions before removing caps. (see General Considerations below).

その他情報

Darzynkiewicz, Z., et al. 1997. Cytometry27, 1.
Frey, T. 1997. Cytometry28, 253.
Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
Gavrieli, Y., et al. 1992. J. Cell Biol.119, 493.
Fawthrop, D. J., et al. 1991. Arch. Toxicol.65, 437.
Martin, S.J., et al. 1990. J. Immunology145, 1859.
Wyllie, A. H. 1980. Nature284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer26, 239.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Parafilm is a registered trademark of Bemis Company, Inc.

安全性情報

• ピクトグラム: GHS08,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H319,H350,H411
• 注意書き: P202 - P273 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B - Eye Irrit. 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects