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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
13C9, monoclonal
化学種の反応性
human
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ARPC3(10094)
詳細
The Arp2/3 complex, which contains ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC, plays a major role in the regulation of the actin polymerization in the cytoskeleton. Two of complex′s seven subunits (ARP2 and ARP3) closely resemble monomeric actin and serve as nucleation sites for new actin filaments. The Arp2/3 complex works in conjunction with an activating nucleation-promoting factor (NPF) to aid in the formation of branched actin networks. The regulation of actin cytoskeleton reorganization is important for processes like cell migration, phagocytosis, and intracellular motility of lipid vesicles.
免疫原
GST-tagged recombinant protein corresponding to human Arp2/3 complex.
アプリケーション
Research Category
細胞骨格
細胞骨格
Anti-Arp2/3 complex Antibody, clone 13C9 detects level of Arp2/3 complex & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected Arp2/3 complex in human colorectal cancer and human colon tissues (antibody may need further dilution if staining is too strong - optimal dilution to be determined by the end user).
Research Sub Category
Cytoskeleton
Cytoskeleton
品質
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Arp2/3 complex on 10 µg of MCF-7 cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Arp2/3 complex on 10 µg of MCF-7 cell lysate.
ターゲットの説明
~20 kDa observed
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
MCF-7 cell lysate
MCF-7 cell lysate
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABT95:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Aida M Lopez-Guerrero et al.
Scientific reports, 10(1), 6580-6580 (2020-04-22)
Tumor invasion requires efficient cell migration, which is achieved by the generation of persistent and polarized lamellipodia. The generation of lamellipodia is supported by actin dynamics at the leading edge where a complex of proteins known as the WAVE regulatory
Diana C Muñoz-Lasso et al.
Scientific reports, 10(1), 5207-5207 (2020-04-07)
Abnormalities in actin cytoskeleton have been linked to Friedreich's ataxia (FRDA), an inherited peripheral neuropathy characterised by an early loss of neurons in dorsal root ganglia (DRG) among other clinical symptoms. Despite all efforts to date, we still do not
Sarah R Barger et al.
Nature communications, 10(1), 1249-1249 (2019-03-21)
Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. For antibody-covered targets, phagocytosis is thought to proceed through the sequential engagement of Fc-receptors on the phagocyte with antibodies on the target
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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