A5979

モノクロナール抗ARP3 マウス宿主抗体

clone FMS338, purified from hybridoma cell culture

• 別名: ARP3 Detection Antibody, Mouse Anti-ARP3
• MDL番号: MFCD04118400
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: FMS338, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 40-45 kDa
• 化学種の反応性: human, mouse, rat, canine
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of mouse myoblast C2 cells
• アイソタイプ: IgG2b
• UniProtアクセッション番号: P61158
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ACTR3(10096) ; mouse ... Actr3(74117) ; rat ... Actr3(81732)

詳細

詳細

Monoclonal Anti-ARP3 (mouse IgG2b isotype) is derived from the hybridoma FMS338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP3. Arp3 is mapped to human chromosome 2q14.1.

免疫原

リコンビナントヒトARP3。

アプリケーション

Monoclonal Anti-ARP3 antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunoblotting
• immunoprecipitation
• immunofluorescence
• immunohistochemistry

生物化学的／生理学的作用

The ARP2/3 complex consists of seven distinct proteins (Arp2 and Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc) that modulate polymerization of cellular actin filaments. ARP3 regulates actin nucleation, and is involved in directing the movement of Listeria monocytogenes in infected host cells. Monoclonal Anti-ARP3 antibody binds to human, canine, rat, and mouse ARP3.

The actin-related protein-2/3 (ARP2/3) is part of Wiskott-Aldrich Syndrome protein (WASP) signaling in mediating polymerization state of actin filaments. It has been found that ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar2 is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable