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製品名
Anti-Integrin alpha 5 (CD49e) Antibody, clone mAb16, clone mAb16, from rat
由来生物
rat
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
mAb16, monoclonal
交差性
human
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ITGA5(3678)
詳細
Integrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on multiple cell types. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 4-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serve as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28).
特異性
Clone mAb16 is a rat monoclonal antibody that specifically detects Integrin alpha5 in human cells.
免疫原
Fibronectin receptor purified from human placenta.
アプリケーション
Research Category
細胞骨格
細胞骨格
Anti-Integrin alpha5 (CD49e), clone mAb16, Cat. No. MABT820, is a rat monoclonal antibody that detects Integrin alpha5 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition assays.
Inhibition Analysis: 10 ug/mL from a representative lot prevented HT-1080 cells from adhering to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
Inhibition Analysis: A representative lot inhibited WI38 fibroblast attachment to fibronectin (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Inhibition Analysis: A representative lot inhibited A375-SM cell spreading. (Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).
Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Inhibition Analysis: A representative lot inhibited WI38 fibroblast attachment to fibronectin (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Inhibition Analysis: A representative lot inhibited A375-SM cell spreading. (Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).
Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
品質
Evaluated by Immunocytochemistry in HFF cells.
Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.
Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.
ターゲットの説明
114.54 kDa calculated.
物理的形状
Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2a in PBS without azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABT820:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Michael J McClure et al.
American journal of physiology. Cell physiology, 316(6), C876-C887 (2019-03-21)
Regenerative medicine treatments for severe skeletal muscle injuries are limited, resulting in persistent functional deficits. Clinical options include neglecting the wound with the expectation that fibrosis will develop or using an autologous muscle graft with minimal functional improvement. A regenerative
Brian J Kwee et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(35) (2021-08-28)
Mesenchymal stromal cells (MSCs) are increasingly combined with biomaterials to enhance their therapeutic properties, including their immunosuppressive function. However, clinical trials utilizing MSCs with or without biomaterials have shown limited success, potentially due to their functional heterogeneity across different donors
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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