MABT411

Anti-Desmocollin-2 Antibody, clone 7G6

clone 7G6, from mouse

• 別名: Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 7G6, monoclonal
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_004940
• UniProtアクセッション番号: Q02487
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DSC3(1825)

詳細

詳細

Desmocollin-2, also known as Desmocollin-3 or Desmosomal glycoprotein II, or Desmosomal glycoprotein III, and encoded by the gene DSC2/CDHF2/DSC3, is a component of intercellular desmosome junctions. Desmocollin-2 is involved in the interaction of plaque proteins and intermediate filaments that mediate cell-cell adhesion and interacts with DSP, PKP2 and JUP proteins. Additionally, Desmocollin-2 is important for epidermal cell positioning and stratification because it mediates the differential adhesiveness between cells that express different isoforms. Desmocollin-2 is localized to the cell membrane at cell junctions and desmosomes. Desmocollin-2 is expressed in epithelia, myocardium and lymph nodes. Defects in Desmocollin-2 may be associated with Familial arrhythmogenic right ventricular dysplasia 11 (ARVD11) a congenital heart disease characterized by infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.

免疫原

Epitope: This antibody recognizes the extracellular domain of Desmocollin-2.

MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2.

アプリケーション

Research Category
細胞骨格

Research Sub Category
ECMタンパク質

Immuonoprecipitation Anlaysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells transfected with C-terminal deletions of plakoglobin retaining 707 amino acids (Wahl, J. K. 3rd, et al. (1996). J Cell Sci. 109(Pt 5):1143-1154.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in Primary human Keratinocytes (Franke, WW., et al (2006). Eur. J. Cell Biol. 85(2); 69-82.)

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells (Wahl, J. K. 3rd. (2005). J Cell Biochem. 96(2):390-403.).

This Anti-Desmocollin-2 Antibody, clone 7G6 is validated for use in western blotting, IP, IHC & ICC for the detection of Desmocollin-2.

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmocollin-2 in 10 µg of A431 cell lysate.

ターゲットの説明

~110 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable