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Key Documents

安全性情報

MABT329M

Sigma-Aldrich

Anti-Cytokeratin 8 Antibody, clone TROMA-1

clone TROMA-1, from rat

別名:

Keratin type II cytoskeletal 8, Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rat

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

TROMA-1, monoclonal

化学種の反応性

human, mouse

包装

antibody small pack of 25 μg

テクニック

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

アイソタイプ

IgG2aκ

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... KRT8(3856)
mouse ... Krt8(16691)

詳細

Keratin, type II cytoskeletal 8 (UniProt: P11679; also known as Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the Krt8 (also known as Krt2-8) gene (Gene ID: 16691) in murine species. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central a-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is a heterotetramer of two type I and two type II keratins. It is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. CK8 together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.

特異性

Clone TROMA-1 detects cytokeratin-8 in human and murine cells.

免疫原

Intermediate filaments extracted from TDM1 Trophoblastoma cells.

アプリケーション

Immunocytochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunocytochemistry applications (O′Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Wang, X., et. al. (2004). J Cell Sci. 117(Pt 1):53-62).

Western Blotting Analysis: A representative lot detected Cytokeratin 8 in Western Blotting applications (Helenius, T.O., et. al. (2016). Cells. 5(3); O′Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 8 in HepG2 cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytokeratin 8 in mouse lung tissue.

Immunoprecipitation Analysis: A representative lot detected Cytokeratin 8 in Immunoprecipitation applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32).

Radioimmunoassay Analysis: A representative lot detected Cytokeratin 8 in Radioimmunoassy applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32).

Immunofluorescence Analysis: A representative lot detected Cytokeratin 8 in Immunofluorescence applications (Helenius, T.O., et. al. (2016). Cells. 5(3); Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Lin, Y.M., et. al. (2008). J Cell Sci. 121(Pt 14):2382-93; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunohistochemistry applications (Szeder, V., et. al. (2003). Dev Biol. 253(2):258-63; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72).
This rat monoclonal Anti-Cytokeratin 8, clone TROMA-1, Cat. No. MABT329, is a rat monoclonal antibody that detects cytokeratin-8 in human and murine cells & is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmunoassay, & WB.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ug/mL of this antibody detected Cytokeratin 8 in 10 µg of HeLa cell lysate.

ターゲットの説明

~54 kDa observed; 54.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABT329-25UG:
MABT329:


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