MABS1683

Anti-UBE3A Antibody, clone 10H7.1

clone 10H7.1, from mouse

• 別名: Ubiquitin-protein ligase E3A, EC: 2.3.2.26, E6AP ubiquitin-protein ligase, HECT-type ubiquitin transferase E3A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 10H7.1, monoclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_000453
• UniProtアクセッション番号: Q05086
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... UBE3A(7337)

詳細

詳細

Ubiquitin-protein ligase E3A (UniProt: Q05086; also known as EC: 2.3.2.26, E6AP ubiquitin-protein ligase, HECT-type ubiquitin transferase E3A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54) is encoded by the UBE3A (also known as E6AP, EPVE6AP, HPVE6A) gene (Gene ID: 7337)in human. UBE3A is the E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified, including the RAD23A and RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. UBE3A catalyzes the high-risk human papilloma virus (HPV) E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Phosphorylation at Tyr659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins and it also promotes its own degradation in vivo. It is reported to plays an important role in the regulation of the circadian clock by ubiquitinating the core clock component ARNTL/BMAL1 and leading to its proteasomal degradation. Three isoforms of UBE3A have been reported that are generated via alternative splicing. Mutations in UBE3A gene cause Angelman syndrome that is characterized by severe motor and intellectual retardation, ataxia, hypotonia, and frequent jerky limb movements.

特異性

Clone 10H7.1 detects Ubiquitin-protein ligase E3A in human cells. it targets an epitope with in 101 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human Ubiquitin-protein ligase E3A.

アプリケーション

Anti-UBE3A, clone 10H7.1, Cat. No. MABS1683, is a mouse monoclonal antibody that detects Ubiquitin-protein ligase E3A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected UBE3A in 10 µg of HeLa cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE3A in human cerebral cortex and human testis tissue.

品質

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UBE3A in 10 µg of PC-12 cell lysate.

ターゲットの説明

~100 kDa observed; 100.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable