MABS1190

Anti-Glucokinase/GCK Antibody, clone 9G1.2

clone 9G1.2, from mouse

• 別名: Glucokinase, GK, Hexokinase-4, Hexokinase-D, Hexokinase type IV, HK IV, HK4
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 9G1.2, monoclonal
• 化学種の反応性: human
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000153
• UniProtアクセッション番号: P35557
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GCK(2645)

詳細

詳細

Glucokinase (EC 2.7.1.2; UniProt P35557; also known as GK, Hexokinase-4, Hexokinase-D, Hexokinase type IV, HK IV, HK4) is encoded by the GCK (also known as HHF3, MODY2) gene (Gene ID 2645) in human. Glucokinase/GK functions as the body′s primary glucose sensor by catalyzing the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, the rate-limiting step of glucose metabolism in human pancreas and liver. GK activity is regulated rapidly in response to the elevated blood glucose after feeding, whereby a change in GK conformation causes a dissociation of the nuclear GK-glucokinase regulatory protein (GKRP) complex, allowing GK translocation to the cytoplasm in an activated state. In mammals, there exist four types of hexokinases, HK1, HK2, and HK3 have high affinity for glucose, while GK (also known as HK4) has low affinity for glucose and its expression is restricted to the pancreas and liver. GCK gene mutations cause Maturity-onset diabetes of the young 2 (MODY2) and Familial hyperinsulinemic hypoglycemia 3 (HHF3).

特異性

Clone 9G1.2 recognizes an epitope present in all three spliced isoforms of human Glucokinase/GCK reported by UniProt (P35557).

免疫原

Epitope: Internal (C-terminal half).

GST-tagged recombinant internal fragment from the C-terminal half of human Glucokinase/GCK.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
グルコース/グリコーゲン代謝

Anti-GlucokinaseAntibody, clone 9G1.2 is an antibody against GCK for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glucokinase/GCK in human liver, colon, and tonsil tissue sections.

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glucokinase/GCK in 10 µg of HepG2 cell lysate.

ターゲットの説明

~52 kDa observed. ~52 kDa observed. 52.19 kDa (isoform 1), 52.13 kDa (isoform 2), and 52.04 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable