MABN59

Anti-ACE2 Antibody, clone 4G5.1

clone 4G5.1, from mouse

• 別名: Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• タグ: GST tagged
• 抗体製品の状態: purified antibody (protein G)
• 抗体製品タイプ: primary antibodies
• クローン: 4G5.1, monoclonal
• 分子量: ~62 kDa
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_068576
• UniProtアクセッション番号: Q9BYF1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ACE2(59272)

詳細

詳細

Angiotensin-converting enzyme 2 (ACE2) is a transmembrane protein belonging to the metallopeptidases (M2) family. It is a membrane-associated carboxypeptidase, expressed in the vascular endothelial cells of the heart, kidney, testis, gastrointestinal system and in the epithelial cells of lungs. ACE2 consists of an N-terminal peptidase domain (PD) and a C-terminal collectrin-like domain (CLD). It also comprises a single catalytic domain that is similar to that of ACE. The gene ACE2 is mapped to human chromosome location Xp22.2. ACE2 is a structural homolog ACE.

特異性

This antibody recognizes ACE2.

免疫原

GST-tagged recombinant protein corresponding to human ACE2.

アプリケーション

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.

ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

生物化学的／生理学的作用

Angiotensin-converting enzyme-2 (ACE2) produces angiotensin (1-9) by hydrolysis of angiotensin 1. Angiotensin (1-9) is a precursor for angiotensin (1-7). ACE2 may also directly produce angiotensin (1-7) by conversion of angiotensin II, which is involved in vasoconstriction.(2) Angiotensin (1-7) plays an important role in the renin-angiotensin system (RAS) signaling pathway and is involved in bradykinin-induced vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as lisinopril and captopril. This gene is upregulated in heart failure due to the overactivity of angiotensin II. ACE2 is studied for its potential therapeutic value for hypertension and heart failure. It binds to the viral envelope protein spike (S) protein and acts as a major entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 in human cells. Hence, it is considered as a potential therapeutic target for COVID-19. ACE2 exhibits protection from lung injury.

品質

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.

ターゲットの説明

~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.

関連事項

Replaces: MAB5676

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HEK293 cell lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable