MABN1833

Anti-nAChR α4 Subunit Antibody, clone 369

clone 369, from mouse

• 別名: Neuronal acetylcholine receptor subunit alpha-4, alpha4 nAChR subunit, Cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 369, monoclonal
• 交差性: monkey, human, rabbit
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin)
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000735
• UniProtアクセッション番号: P43681
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CHRNA4(1137)

詳細

詳細

Neuronal acetylcholine receptor subunit alpha-4 (UniProt P43681; also known as alpha4 nAChR subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit) is encoded by the CHRNA4 (also known as NACRA4, NACHRA4) gene (Gene ID 1137) in human. The nicotinic acetylcholine (ACh) receptors (nAChRs) are activated by acetylcholine (ACh) and nicotinic agonists, such as cytisine, 1,1-dimethyl-4-phenylpiperazinium and carbachol. The nAChRs are ligand-gated pentameric cation channels. Currently, 12 neuronal and 5 muscle nAChR subunits have been identified. The nAChRs can exist as homopentamers of five identical alpha subunits (α7, α8, or α9), alpha heteropentamers composed of more than one type of alpha subunits (α7-α10), or as alpha/beta heteropentamers consisting of various combinations of alpha (α2-α6) and beta (β2-β4) subunits. The heteromeric alpha/beta types display high ligand affinity, but are insensitive to α-bungarotoxin (αBgt), while the alpha-only homomeric and heteromeric pentamers show lower agonist affinity, but high αBgt sensitivity. The α4β2 (two α4 and three β2 subunits) and α7 (five α7 subunits) nAChRs are the predominant nAChRs in the central nervous system (CNS) among mammalian species. The alpha-4 subunit is initially produced with a signal peptide (a.a. 1-28), the removal of which yields the mature 627-amino acid membrane protein with a large extracellular domain (a.a. 29-242), four transmembrane segments (a.a. 243-267, 275-293, 309-330, 601-619), and a large cytoplasmic loop (a.a. 331-600) between the third and fourth transmembrane domains.

免疫原

Recombinant human nAChR α4 subunit.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経伝達物質及び受容体

Anti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.

Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected nAChR α4 Subunit in human small intestine tissue.
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in monkey frozen retina sections by fluorescence immunohistochemistry (Liu, J., et al. (2009). Invest. Ophthalmol. Vis .Sci. 50(3):1408-1415).
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in various areas of the rabbit brainstem by fluorescence immunohistochemical staining of free-floating sections (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Immunofluorescence Analysis: A represenative lot detected an increase of nAChR α4-positive neurons in the A1, A2 and A6 brainstem noradrenergic areas of female rabbits 1 hr after mating by fluorescence immunohistochemical staining of free-floating sections. The number of nAChR α4-positive neurons subsided to basal level 2 hrs after mating (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).

品質

Evaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected nAChR α4 Subunit in human cerebellum tissue.

ターゲットの説明

67.16 kDa calculated.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable