MABN1598

Anti-RetGC-1/GUCY2D Antibody, clone 6D8.2

clone 6D8.2, from mouse

• 別名: Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, RETGC-1, Rod outer segment membrane guanylate cyclase, ROS-GC
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 6D8.2, monoclonal
• application: WB
• 化学種の反応性: human
• テクニック: western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 6D8.2, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000171
• UniProtアクセッション番号: Q02846
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GUCY2D(3000)

詳細

詳細

Retinal guanylyl cyclase 1 (EC 4.6.1.2; UniProt Q02846; also known as Guanylate cyclase 2D, retinal, RETGC-1, Rod outer segment membrane guanylate cyclase, ROS-GC) is encoded by the GUCY2D (also known as CORD6, GUC1A4, GUC2D, RETGC, RETGC1) gene (Gene ID 3000) in human. RetGC-1 is a membrane-bound retinal guanylyl cyclase expressed predominantly in the cone and rod photoreceptors in the cone outer segment. RetGC-1 and the related RetGC-2 mediate the synthesis of cyclic 3′, 5′-guanosine monophosphate (cGMP) from guanosine triphosphate in mammalian photoreceptor cells, where RetGC-1 and its associated activator proteins are responsible for the Ca2+-sensitive restoration of cGMP levels after light activation of the phototransduction cascade. RetGC-1 is produced with a signal peptide (a.a. 1-51) sequence, the removal of which yields the mature enzyme with an exracellular domain (a.a. 52-462), followed by a transmembrane segment (a.a. 463-487) and a cytoplasmic tail (a.a. 488-1103) that contains a protein kinase homology domain (a.a. 525-808), a dimerization domain, and the guanylate cyclase catalytic domain (a.a. 880-1010). Heterozygous mutations in the GUCY2D gene have been shown to cause autosomal dominant cone and cone-rod dystrophies (adCODs and adCORDs, respectively), while homozygous or compound heterozygous mutations cause autosomal recessively inherited Leber Congenital Amaurosis (LCA), the most severe form of inherited retinopathy, resulting in total blindness or greatly impaired vision at birth or in early infancy.

特異性

Clone 6D8.2 targets an epitope in the cytoplasmic kinase domain of human retinal guanylyl cyclase 1 (RetGC-1) encoded by the GUCY2D gene.

免疫原

Epitope: Kinase domain.

GST-tagged recombinant human RetGC-1/GUCY2D kinase domain fragmen.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
感覚・末梢神経

This Anti-RetGC-1 Antibody, clone 6D8.2 is validated for use in Western Blotting for the detection of GUCY2D.

品質

Evaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RetGC-1/GUCY2D in 10 µg of Y79 human retinoblastoma cell lysate.

ターゲットの説明

~120 kDa observed. 114.7/120.1 kDa (mature/pro-form) calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1