MABN866

Anti-Myocilin (NT) Antibody, clone 7.1

clone 7.1, from mouse

• 別名: Myocilin, Myocilin 55 kDa subunit, Trabecular meshwork-induced glucocorticoid response protein, Myocilin, N-terminal fragment, Myocilin 20 kDa N-terminal fragment
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 7.1, monoclonal
• 化学種の反応性: human, mouse
• テクニック: immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000252
• UniProtアクセッション番号: Q99972
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MYOC(4653)

詳細

詳細

Myocilin (mutated trabecular meshwork-induced glucocorticoid response protein) is encoded by the MYOC gene (also known as GLC1A, GPOA, JOAG, JOAG1, TIGR) in human (Entrez Gene ID 4653). Myocilin is produced as a 504 amino acid glycoprotein containing an N-terminal hydrophobic signal peptide sequence, a coiled-coiled leucine zipper domain, and a C-terminal domain with homology to olfactomedins. Myocilin is commonly identified as a 53/57 kDa doublet by gel electrophoresis, and a 66 kDa form of the protein is also reported. Myocilin is known to undergo intracellular endoproteolytic cleavage between Glu214 and Leu215, resulting in a 20 kDa N-terminal and a 35 kDa C-terminal fragment. Myocilin is identified as a Lingo-1 receptor ligand and MYOC gene mutations are linked to 10% of juvenile open-angle glaucoma cases and 3-4% of those with primary open-angle glaucoma (PMID 24732711 24741044, 24837143). Cat. No. MABN866, clone 7.1 is a mouse monoclonal antibody that recognizes an epitope within the N-terminal fragment (aa33-214) and is demonstrated to be suitable for Western blotting, immuncytochemistry, and immunhistochemistry applications. This clone is reactive toward both human and murine species. (PMID 18674535 & 23979599).

免疫原

Epitope: N-terminal fragment (aa 33-214)

Recombinant protein corresponding to the N-terminal fragment (aa 33-214) of human Myocilin.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
発生シグナル伝達

Anti-Myocilin Antibody, clone 7.1 is an antibody against Myocilin for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in aqueous humor (Zhao, Y., et al. (2013). Mol. Cell Biol. 33(21):4225-4240).

品質

Evaluated by Western Blotting in mouse eye tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Myocilin in 10 µg of mouse eye tissue lysate.

ターゲットの説明

~53, 60 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable