MABF3136

Anti-IL-11 Antibody, clone 13-6D-10G

• 別名: Interleukin-11
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rat
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 13-6D-10G, monoclonal
• 分子量: calculated mol wt 21.52 kDa; observed mol wt ~20 kDa
• 精製方法: using protein G
• 化学種の反応性: mouse
• 包装: antibody small pack of 100 μL
• テクニック: inhibition assay: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_032376
• UniProtアクセッション番号: P47873
• 保管温度: -10 to -25°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Il11(16156)

詳細

詳細

Interleukin-11 (UniProt: P47873 ; also known as IL-11) is encoded by the Il11 gene (Gene ID: 16156) in murine species. IL-11 is member of the IL-6 family of cytokines that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce mature secreted protein. It is involved in a variety of biological responses, including hematopoiesis, bone development, and carcinogenesis. Its actions are mediated via interaction with IL-11 receptor that is composed of IL-11R and gp130. Binding of IL-11 to its receptor induces the activation of JAK that phosphorylates STAT3, which then translocates to the nucleus and activates transcription of various genes. IL-11 is reported to promote the proliferation of hepatocytes in response to liver damage. In mice subjected to liver injury, IL-11 is mainly produced by hepatocytes in response to reactive oxygen species (ROS) released from dying hepatocytes. Secreted IL-11 then induces STAT3 phosphorylation in adjacent healthy hepatocytes resulting in their compensatory proliferation. It has been reported that IL-11 production in dying hepatocytes depended on ERK2 that phosphorylates Fra-1 and prevents its proteasomal degradation. Fra-1 is subsequently recruited to the Il11 promoter and activates transcription. Clone 13-6D-10G (13) displays neutralizing activity against mIL-11. It blocks mIL-11-induced, but not mIL-6-induced the proliferation of T1165 cells. Preincubation of murine IL-11 with this clone is shown to completely inhibit STAT3 phosphorylation. (Ref.: Deguchi, Y., et al. (2018). Biochem. Biophys. Res. Commun. 505(2); 453-459; Nishina, T., et al. (2012). Sci. Signal. 5(207); ra5).

特異性

Clone 13-6D-10G is a rat monoclonal antibody that detects murine IL-11.

免疫原

FLAG-tagged full-length recombinant murine IL-11 produced in HEK293T cells transfected with pcDNA3-mIl11.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HEK293T cells transiently transfected with mIL-11.

Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected IL-11 in HEK293T cells transiently transfected with mIL-11, but not in wild-type HEK293T cells.

Tested Applications

Inhibition Assay: A representative lot inhibited mIL-11 induced STAT3 phosphorylation and T1165 cell proliferation. (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459).

Western Blotting Analysis: A representative lot detected IL-11 in Western Blotting applications (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

物理的形状

Purified rat monoclonal antibody IgG1 in PBS without azide.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable