A1231

モノクローナル抗オーロラ-Aキナーゼ抗体 マウス宿主抗体

clone 35C1, purified from hybridoma cell culture

• 別名: モノクロナール抗オーロラ-Aキナーゼ, 抗ARK-1抗体, 抗AURORA2抗体, 抗BTAK抗体, 抗STK15抗体, 抗STK6抗体, 抗オーロラ関連キナーゼ1抗体, 抗セリン/スレオニンプロテインキナーゼ15抗体
• MDL番号: MFCD06411780
• UNSPSCコード: 12352203
• eCl@ss: 32160410
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in mouse cell line
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 35C1, monoclonal
• 形状: PBS solution
• 分子量: antigen ~46 kDa
• 化学種の反応性: human, mouse
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using HeLa total cell extract
• アイソタイプ: IgG2b
• UniProtアクセッション番号: O14965
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... AURKA(6790) , AURKA(6790) ; mouse ... Aurka(20878) , Aurka(20878)

詳細

詳細

Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome.

特異性

Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.

免疫原

リコンビナントヒトオ-ロラ-Aキナ-ゼ。

アプリケーション

Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry.

Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.

生物化学的／生理学的作用

Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans .

Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr¹⁵ by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser³⁵³. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)