MABF2186

Anti-Zika Virus NS1 protein Antibody

purified antibody, clone 680, from mouse

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 680, monoclonal
• 分子量: calculated mol wt 47 kDa
• 化学種の反応性: virus (Zika)
• 包装: antibody small pack of 100 μg
• テクニック: ELISA: suitable
• アイソタイプ: IgG
• 輸送温度: dry ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

特異性

Clone 680 is a mouse monoclonal antibody that detects Zika virus NS1 protein.

免疫原

His-tagged recombinant Zika Virus NS1 protein expressed in mammalian cells.

アプリケーション

Quality Control TestingEvaluated by ELISA with recombinant Zika virus NS1 antigen.ELISA Analysis: 1 μg/mL of this antibody detected recombinant Zika Virus NS1 protein.

Anti-Zika Virus NS1 protein, clone 680, Cat. No. MABF2186, is a mouse monoclonal antibody that detects Zika Virus NS1 protein and is tested in ELISA.

ターゲットの説明

Genome polyprotein (UniProt: Q32ZE1) following its synthesis is cleaved into 13 different proteins in Zika virus. Zika virus is a member of the Flaviviridae family that contains a single positive sense RNA genome. Following the fusion of virus membrane with host endosomal membrane, RNA genome is released into the cytoplasm. It is initially translated as a single polyprotein, which is post-translationally cleaved into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins. Six of the NS proteins (NS2A to NS5) are shown to form a replication complex on the cytoplasmic side of the endoplasmic reticulum membrane. The glycosylated NS1 that is reported to associates with lipids, forms a homodimer inside the cells and is shown to be essential for viral replication and late in infection. It is also secreted into the extracellular space as a hexameric lipoprotein particle and plays a role in immune evasion and pathogenesis via its interaction with components from both the innate and adaptive immune systems. NS1 is also a major antigenic marker for viral infection. NS2A functions in virion assembly and antagonizes the host immune response. The non-structural protein 2B is essential for the serine protease function of NS3. The serine protease NS3 has three different enzymatic activities - serine protease, NTPase and RNA helicase. The non-structural protein 4A of virus regulates the ATPase activity of the NS3 helicase while the non-structural protein 4B inhibits interferon-induced host STAT1 phosphorylation and its nuclear translocation. (Ref.: Song, H. et al. (2016). Nat. Struct. Mol. Biol. 23(5); 456-458; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at +2°C to +8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable