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Key Documents

MABF2052

Sigma-Aldrich

Anti-Chikungunya virus Antibody, clone 3E7b

clone 3E7b, from mouse

別名:

CHIKV

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.43

由来生物

mouse

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

3E7b, monoclonal

化学種の反応性

virus

包装

antibody small pack of 25 μL

テクニック

ELISA: suitable
immunocytochemistry: suitable
neutralization: suitable
western blot: suitable

アイソタイプ

IgMκ

ターゲットの翻訳後修飾

unmodified

詳細

Chikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. This monoclonal antibody (3E7b) can bind strongly to native CHIKV surface and potently neutralize virus replication (Ref.: Marsinoul, P., et al. (2014). Virology 464-465; 111-117; Lam, S., et al. (2015). mAbs 7(6), 1178-1194).

特異性

Clone 3E7b detects Chikungunya virus in cells infected with this virus.

免疫原

Chikungunya virus

アプリケーション

Research Category
炎症及び免疫
Anti-Chikungunya virus, clone 3E7b, Cat. No. MABF2052, is a mouse monoclonal antibody that detects E2 protein of Chikungunya virus and has been tested for use in ELISA, Immunocytochemistry, Neutralizing asssay, and Western Blotting.
Western Blotting Analysis: A representative lot detected Chikungunya virus in Western Blotting applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

Immunocytochemistry Analysis: A representative lot detected Chikungunya virus in Immunocytochemistry applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Chikungunya virus in BHK cells infected with Chikungunya virus. (Courtesy from an independent lab).

Neutralizing Analysis: A representative lot neutralized Chikungunya virus in Neutralizing applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

ELISA Analysis: A representative lot detected Chikungunya virus in ELISA applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

品質

Isotype testing: Identity Confirmation by Isotyping Test.

Isotype Analysis: The identity of this monoclonal antibody is confirmed by isotype test to be mouse IgMk.

物理的形状

Format: Purified
Purified mouse monoclonal antibody IgM in PBS without azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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