MABE227

Anti-FTO Antibody, clone 5-2H10

clone 5-2H10, from mouse

• 別名: Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 5-2H10, monoclonal
• 交差性: human, rat
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001073901
• UniProtアクセッション番号: Q9C0B1
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FTO(79068)

詳細

詳細

Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.

免疫原

Recombinant protein corresponding to human FTO.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Anti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected FTO in glandular cells of rat pituitary gland tissue.

品質

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FTO in 10 µg of human kidney tissue lysate.

ターゲットの説明

~58 kDa observed.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Human kidney tissue lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable