MABE1015

Anti-CST complex subunit CTC1 Antibody, clone 8E5.2

clone 8E5.2, from mouse

• 別名: CST complex subunit CTC1, Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 8E5.2, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_079375
• UniProtアクセッション番号: Q2NKJ3
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CTC1(80169)

詳細

詳細

CST complex subunit CTC1 (UniProt Q2NKJ3; also known as Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B) is encoded by the CTC1 (also known as C17orf68, CRMCC) gene (Gene ID 80169) in human. CTC1 is a subunit of the trimeric CST (CTC1, STN1, and TEN1) complex that binds ssDNA, promotes telomere DNA synthesis, and inhibits telomerase-mediated telomere elongation. CST directly interacts with and stimulates DNA polymerase α-primase (polα-primase) activity. CST also associates with and sequesters telomeric 3′ overhangs after their elongation by telomerase, thereby limiting further telomere extension by telomerase. Knockdown of either STN1 or CCT1 in human cancer cells and in mouse fibroblasts results in substantially elongated telomeric G-overhangs. Conditional deletion of CTC1 in mice leads to early entry into senescence and signs of telomeric replication defects, Human CTC1 gene mutations casue dyskeratosis congenita (DC) and related bone marrow failure syndromes, as well as the rare autosomal recessive neurological disorder Coats Plus, where patients develop cranial calcifications and cysts. Coats Plus and DC patients develop premature hair graying, anemia, and osteoporosis as a result of telomerase defects.

特異性

Expected to react with both spliced isoforms of human CTC1 reported by UniProt (Q2NKJ3).

免疫原

Epitope: Internal (C-terminal half).

GST-tagged recombinant internal fragment from the C-terminal half of human CTC1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Anti-CST complex subunit CTC1 Antibody, clone 8E5.2 is an antibody against CST complex subunit CTC1 for use in Western Blotting.

品質

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CST complex subunit CTC1 in 10 µg of human cortex tissue lysate.

ターゲットの説明

~135 kDa observed. 134.6 kDa (isoform 1) and 114.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable