ABC225

Anti-phospho USP7 (Ser18) Antibody

from rabbit, purified by affinity chromatography

• 別名: Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• テクニック: inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_003461
• UniProtアクセッション番号: Q93009
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer18)
• 遺伝子情報: human ... USP7(7874)

詳細

詳細

Ubiquitin-specific-processing protease 7 (USP7) is also known as Ubiquitin carboxyl-terminal hydrolase 7 or Herpesvirus-associated ubiquitin-specific protease (HAUSP). USP7 is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, USP7 activity generally stabilizes its substrate proteins, and it is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.

免疫原

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human USP7 phosphorylated at Ser18.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

Peptide Inhibition Analysis: 0.2 µg/mL from a representative lot was blocked by a phospho-peptide in HeLa cell lysate.

Western Blotting Analysis: 0.02 µg/mL from a representative lot detected phospho USP7 (Ser18) in HeLa cell lysate (Prof. Grigory Dianov, University of Oxford, U.K.).

Western Blotting Analysis: 0.02 µg/mL from a representative lot detected 100 ng of phospho USP7 (Ser18), and not dephosphorylated USP7 (Prof. Grigory Dianov, University of Oxford, U.K.).

Alexa Fluor^™ is a registered trademark of Life Technologies.

This Anti-phospho USP7 (Ser18) antibody is validated for use in western blotting & peptide inhibition assay for the detection of phospho USP7 (Ser18).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected phospho USP7 (Ser18) in 10 µg of HeLa cell lysate.

ターゲットの説明

~140 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable