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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
2A8.2, monoclonal
化学種の反応性（ホモロジーによる予測）
all
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG2b
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... NOS1(4842)
詳細
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
特異性
Reacts with nitrotyrosine.
免疫原
Nitrated KLH.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
酸化ストレス
酸化ストレス
Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody, clone 2A8.2 validated for use in IH(P) & WB.
Immunohistochemistry:
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
品質
Evaluated by Western Blot on Nitrotyrosine Immunoblot Controls.
Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
物理的形状
Protein A purified
Format: Purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.1% sodium azide.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
Nitrated BSA
Nitrated BSA
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB5404:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Epitope motif of an anti-nitrotyrosine antibody specific for tyrosine-nitrated peptides revealed by a combination of affinity approaches and mass spectrometry.
Mihaela Dr Gu?anu,Brindu?a-Alina Petre,Michael Przybylski
Journal of Peptide Science null
A Kiss et al.
British journal of pharmacology, 155(7), 1015-1024 (2008-10-11)
Exogenous peroxynitrite from nanomolar to micromolar concentrations exerts cardioprotection. Here, we have assessed its effects on ischaemia- and reperfusion-induced ventricular arrhythmias in vivo and a possible role for mitochondrial K(ATP) channels in these effects, using the channel inhibitor 5-hydroxydecanoate (5-HD).
Ben C Creelan et al.
OncoTargets and therapy, 10, 4239-4250 (2017-09-19)
Omaveloxolone is a semisynthetic oleanane triterpenoid that potently activates Nrf2 with subsequent antioxidant function. We conducted a first-in-human Phase I clinical trial (NCT02029729) with the primary objectives to determine the appropriate dose for Phase II studies, characterize pharmacokinetic and pharmacodynamic
When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins.
Petre, BA; Ulrich, M; Stumbaum, M; Bernevic, B; Moise, A; Doring, G; Przybylski, M
Journal of the American Society For Mass Spectrometry null
Deficiency of the Bax gene attenuates denervation-induced apoptosis.
P M Siu, S E Alway, P M Siu, S E Alway
Apoptosis null
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