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Merck
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安全性情報

MAB5326C3

Sigma-Aldrich

Anti-Nestin Antibody, clone 10C2, Cy3 conjugate

clone 10C2, from mouse, CY3 conjugate

別名:

Nestin10c2, NES, Nbla00170, FLJ21841

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
結合体:
CY3 conjugate
application:
ICC
クローン:
10C2, monoclonal
化学種の反応性:
human
citations:
1
テクニック:
immunocytochemistry: suitable

由来生物

mouse

品質水準

結合体

CY3 conjugate

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

10C2, monoclonal

化学種の反応性

human

テクニック

immunocytochemistry: suitable

アイソタイプ

IgG1

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... NES(10763)

詳細

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

特異性

This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.

免疫原

Fusion protein corresponding to human Nestin.

アプリケーション

Research Category
幹細胞研究

ニューロサイエンス
Research Sub Category
ニューロフィラメント及び神経細胞代謝

神経細胞及びグリアマーカー
Detect Nestin using this Anti-Nestin Antibody, clone 10C2, Cy3 conjugate validated for use in ICC.

品質

Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).

ターゲットの説明

The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa

物理的形状

Protein A purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

保管および安定性

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

アナリシスノート

Control
RNcell CX neural stem cells (SCC007).

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MAB5326C3:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

C A Messam et al.
Experimental neurology, 161(2), 585-596 (2000-02-25)
The presence of the intermediate filament protein nestin has been the predominant marker used to describe stem and progenitor cells in the mammalian CNS. In this study, a 998-bp fragment in the 3' region of the nestin mRNA was cloned
Jinte Middeldorp et al.
Development (Cambridge, England), 137(2), 313-321 (2009-12-31)
A subpopulation of glial fibrillary acidic protein (GFAP)-expressing cells located along the length of the lateral ventricles in the subventricular zone (SVZ) have been identified as the multipotent neural stem cells of the adult mammalian brain. We have previously found
Raelene Endersby et al.
Cancer research, 71(12), 4106-4116 (2011-04-22)
The AKT family, comprising three highly homologous kinases, is an essential mediator of the PTEN/PI3K pathway, which is deregulated in many human cancers. A thorough understanding of the specific activities of each isoform in normal and disease tissues is lacking.
Tania Aguado et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(5), 1551-1561 (2006-02-03)
Endocannabinoids exert an important neuromodulatory role via presynaptic cannabinoid CB1 receptors and may also participate in the control of neural cell death and survival. The function of the endocannabinoid system has been extensively studied in differentiated neurons, but its potential
Teresa Coccini et al.
Current protocols, 2(4), e423-e423 (2022-04-27)
Neurotoxicity (NT) testing for regulatory purposes is based on in vivo animal testing. There is general consensus, however, about the need for the development of alternative methodologies to allow researchers to more rapidly and cost effectively screen large numbers of

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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