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Key Documents

安全性情報

MAB2019Z

Sigma-Aldrich

Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free

clone 15F11, Chemicon®, from mouse

別名:

MAB2019

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

15F11, monoclonal

化学種の反応性

human

メーカー/製品名

Chemicon®

テクニック

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... ITGAV(3685)

特異性

Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.

免疫原

Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.

アプリケーション

Research Category
細胞骨格
Research Sub Category
インテグリン
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.
Immunoprecipitation

Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.

Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.

ELISA

Optimal working dilutions must be determined by end user.

物理的形状

Format: Purified
Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives.

保管および安定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MAB2019Z:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Khine Kyaw Oo et al.
BMC cancer, 21(1), 65-65 (2021-01-16)
Chemoresistance is one of the main problems in treatment of cancer. Periostin (PN) is a stromal protein which is mostly secreted from cancer associated fibroblasts in the tumor microenvironment and can promote cancer progression including cell survival, metastasis, and chemoresistance.
Characterization of monoclonal antibodies against integrin alpha V beta 5.
Stuiver, I and Smith, J W
Hybridoma, 14, 545-550 (1995)
Divalent cations regulate the organization of integrins alpha v beta 3 and alpha v beta 5 on the cell surface.
Stuiver, I, et al.
Journal of Cellular Physiology, 168, 521-531 (1996)
Jim O'Prey et al.
Journal of virology, 82(12), 5933-5939 (2008-04-04)
Adenoviruses for gene or oncolytic therapy are under development. Notable among these strategies is adenoviral delivery of the tumor suppressor p53. Since all therapeutics have limitations in certain settings, we have undertaken retroviral suppressor screens to identify genes conferring resistance
L1 stimulation of human glioma cell motility correlates with FAK activation.
Yang, M; Li, Y; Chilukuri, K; Brady, OA; Boulos, MI; Kappes, JC; Galileo, DS
Journal of Neuro-Oncology null

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