ABT1347

Anti-Neurocan Antibody, C-terminal

from rabbit

• 別名: Neurocan core protein, 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: unpurified
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse
• 包装: antibody small pack of 25 μL
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable; radioimmunoassay: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_113841 (neurocan core protein precursor)
• UniProtアクセッション番号: P55067
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Ncan(58982)

詳細

詳細

Neurocan core protein (UniProt: P55067; also known as 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3) is encoded by the Ncan (also known as Cspg3) gene in rat. Neurocan is a secreted protein of the aggrecan/versican proteoglycan family that is found in early postnatal and adult brain. During early postnatal development neurocan accounts for at least 20% of the protein of the soluble brain proteoglycans. Neurocan mostly contains chondroitin sulfate, but also has N-linked and O-linked oligosaccharides. It is shown to be involved in the modulation of cell adhesion and migration and regulates neurite outgrowth. Neurocan interact with several cell surface molecules, such as N-CAM, L1/Ng-CAM, etc., and modulates the cell-binding and neurite outgrowth promoting activates of these molecules. Neurocan is synthesized with a 22 aa signal peptide that is subsequently cleaved to produce the mature form. Neurocan contains one C-type lectin domain, two EGF-like domains, one Ig-like V type domain, two link domains and one Sushi (CCP/SCR) domain. (Ref.: Rauch, U., et al. (2001) Cell Mol. Life Sci. 58(12-13):1842-56; Zhou, XH et al. (2001). Mol. Cell Biol. 21(17): 5970-78).

特異性

This rabbit polyclonal antibody detects Neurocan in fetal and adult rat brain.

免疫原

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 212 amino acids from the C-terminal half of rat Neurocan core protein.

アプリケーション

Research Category
細胞骨格

Anti-Neurocan, C-terminal, Cat. No. ABT1347, is a rabbit polyclonal antibody that detects Neurocan core protein and has been tested for use in Immunofluorescence, Immunohistochemistry, and Radioimmunoassay.

Radioimmunoassay Analysis: A representative lot detected Neurocan in rat brain (Milev, P., et. al. (1998). Biochem Biophys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Neurocan in E13 and E14 mouse ventral diencephalon and retina in C57 mouse embryos (Leung, K.M., et. al. (2004). Brain Res Dev Brain Res. 152(1):1-10).

品質

Evaluated by Immunofluorescence in E14 rat embryo, E14 rat neocortex, and adult rat brain hippocampus cells.

Immunofluorescence Analysis: A 1:500 dilution of this antibody detected Neurocan in E14 rat embryo, E14 rat neocortex, and adult rat brain hippocampus cells.

ターゲットの説明

135.55 kDa calculated.

物理的形状

Unpurified

Format: Unpurified

Rabbit polyclonal antiserum with 0.02% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable