N0913

Anti-Neurocan antibody, Mouse monoclonal

clone 650.24, purified from hybridoma cell culture

• 別名: Anti-CSPG3, Anti-Chondroitin Sulfate Proteogycan 3
• MDL番号: MFCD02263808
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 650.24, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 260 kDa (additional band at 160 kDa)
• 化学種の反応性: rat
• 包装: antibody small pack of 25 μL
• 濃度: ~1 mg/mL
• テクニック: immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase.
• アイソタイプ: IgG1
• UniProtアクセッション番号: P55067
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Ncan(58982)

詳細

詳細

Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).

Neurocan is a proteoglycan which is expressed in the nervous tissue.

免疫原

embryonic rat brain proteoglycans.

アプリケーション

Anti-Neurocan antibody, Mouse monoclonal has been used in:

• western blotting
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunohistochemistry

生物化学的／生理学的作用

Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction.

Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons.

ターゲットの説明

Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable