SAB4200500

Anti-Collagen IV antibody, Mouse monoclonal

clone J3-2, purified from hybridoma cell culture

• 別名: Anti-COL4, Monoclonal Anti-Collagen IV antibody produced in mouse
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: J3-2, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: mouse, rat, dog, bovine, human, monkey
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: 2.0-4.0 μg/mL using human liver tissue sections.; western blot: 1.0-2.0 μg/mL using human placenta extracts.
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... COL4A1(1282)

詳細

詳細

Human collagen IV comprises six genes. The COL4A1-COL4A2 is mapped to chromosome 13. The COL4A3-COL4A4 and COL4A5-COL4A6 are localized on chromosome 2 and chromosome X, respectively. It belongs to the collagen superfamily and is associated with the basement membranes (BMs). The six α-chains namely α1(IV) to α6(IV) comprises an N-terminal cysteine and lysine domain, a typical Gly-X-Y triple repeats and a C-terminal noncollagenous (NC1) domain.

免疫原

placenta preparation rich in basement membrane collagen.

アプリケーション

Monoclonal Anti-Collagen IV antibody produced in mouse has been used:

• in the immunohistochemical staining
• in the immunostaining
• in the immunofluorescence detection
• as a secondary antibody in the western blot analysis

生物化学的／生理学的作用

Collagen IV plays a key role in basement membrane assembly. The six chains assemble as three different protomers namely, the α 1.α1.α2(IV), α3.α4.α5(IV) and α 5.α5.α6(IV). The trimers are formed in different combinations of the α-chains. High expression levels of α3, α4 and α5 is observed in specialized glomerular BM (GBM). Mutations in the COlA5 gene is associated with Alport syndrome and α3(IV) chain is associated pathogenesis of Goodpasture syndrome, an autoimmune disease. Mutation in the COlA5 is also implicated in Diffuse Esophageal Lewmyomatosis.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable