ABN66

Anti-Cholesterol acyltransferase 1 Antibody

from rabbit, purified by affinity chromatography

• 別名: Sterol O-acyltransferase 1, Acyl-coenzyme A:cholesterol acyltransferase 1, ACAT-1, Cholesterol acyltransferase 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rhesus macaque, human
• 化学種の反応性（ホモロジーによる予測）: rhesus monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rat (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_003092
• UniProtアクセッション番号: P35610
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SOAT1(6646)

詳細

詳細

Cholesterol acyltransferase 1 (ACAT1) is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer′s disease.

特異性

Other homologies: Mouse (93% sequence homology).

免疫原

KLH-conjugated linear peptide corresponding to human Cholesterol acyltransferase 1.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cholesterol acyltransferase 1 in human adrenal gland and normal human brain tissues.

This Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.

品質

Evaluated by Western Blot in Caco-2 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Cholesterol acyltransferase 1 on 10 µg of Caco-2 cell lysate.

ターゲットの説明

~58 kDa observed which may be the result of dimerization/aggregation of the native protein during sample preparation for SDS-PAGE. The calculated molecular weight is 65 kDa

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Caco-2 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable